Investigation of the Effect of 4-Hydroxyphenylboronic Acid on Endothelial Damage due to LPS-Induced Endotoxemia in the HUVEC Line via Endocan

Objective: In our study, the effect of 4-hydroxy phenylboronic acid (4OHFBA), a derivative of boronic acid, on lipopolysaccharide (LPS) induced endothelial damage was investigated in the human umbilical vein endothelial cell line (HUVEC). It has been shown that 4 hydroxy phenylboronic acid may be effective in LPS induced cell death and endothelial damage.
Methods: The study was conducted on the HUVEC line. The cells were treated with 10 μg/mL LPS for 12 h. They were treated with 4 different doses of 4OHFBA 1 h after LPS administration. Cell viability percentage and endocan levels were measured after 24th and 48th hours.
Results: When the endocan levels were examined at 24th and 48th hours, it was observed that the endocan level in the LPS group was significantly higher compared to the healthy group (p<0.05). The endocan levels were significantly lower in the groups treated with 31.25 μM, 62.5 μM, 125 μM, and 250 μM 4OHFBA compared to the LPS group (p<0.05). It is seen that this decrease is much greater in the 62.5 μM + 4OHFBA group and this is the group that is closest to the healthy group (p<0.05).
Conclusion: These findings show that 4OHFBA acid may be effective for LPS-induced cell death and endothelial damage.