Abstract

INTRODUCTION: Folic acid, an important factor in the recovery, and repair of endothelial damage, is involved in the synthesis of building block molecules. In this study, the effect of folic acid on improving endothelial injury was investigated.

METHODS: Repair of endothelial cell damage was measured in real time using real-time live cell imaging system xCELLigence RTCA on HUVEC cell line. The effect of different concentrations of folic acid and folic acid inhibitors on cell proliferation was investigated. All data were statistically analyzed by using the SPSS for Windows V.15.0.

RESULTS: In the standard in vitro wound healing model, it was observed that folic acid had a proliferative effect on endothelial cells. Folic acid also has an enhancing effect on cell migration. In the study, the rate of wound healing increased in the presence of folic acid.

DISCUSSION AND CONCLUSION: : In a study of seven different concentrations of folic acid (2.5-100μM), the dose of folic acid (EC 90 dose) increased by 90% with respect to control cells grown by culture medium to 100 μM; EC 50 dose was found to be 25 μM. Cell proliferation recorded for 48 hours was plotted as time as cell index (CI). The effect of folic acid on endothelial damage repair was demonstrated by the decrease in the presence of folic acid inhibitors.